publications

2026

  1. Environmental gradients and habitat specificity structure benthic microbial assemblages in a temperate seagrass ecosystem
    Gabriel A Serrano, Alejandro De Santiago, Tiago José Pereira, Mirayana Marcelino-Barros, and Holly M Bik
    bioRxiv, 2026

2025

  1. Pseudoalteromonas is a novel symbiont of marine invertebrates that exhibits broad patterns of phylosymbiosis
    Alejandro De Santiago, Shelby Barnes, Tiago José Pereira, Mirayana Marcellino-Barros, Lekeah Durden, Min K Han, J Cameron Thrash, and Holly M Bik
    bioRxiv, 2025
  2. STREAMS guidelines: standards for technical reporting in environmental and host-associated microbiome studies
    Julia M Kelliher, Chloe Mirzayi, Sarah R Bordenstein, Aaron Oliver, Christina A Kellogg, Eneida L Hatcher, Maureen Berg, Petr Baldrian, Mashael Aljumaah, Cassandra Maria Luz Miller, STREAMS Consortium, and others
    Nature Microbiology, 2025
  3. A roadmap for equitable reuse of public microbiome data
    Laura A Hug, Roland Hatzenpichler, Cristina Moraru, André Soares, Folker Meyer, Anke Heyder, The Data Reuse Consortium, and Alexander J Probst
    Nature Microbiology, 2025
  4. Complete genome sequences of two Pseudoalteromonas undina strains isolated from a marine nematode (Oncholaimidae) collected at Tybee Island
    Alejandro De Santiago, Shelby J Barnes, Tiago José Pereira, Mirayana Marcelino-Barros, Holly M Bik*, and J Cameron Thrash*
    Microbiology Resource Announcements, 2025
    * co-corresponding authors
  5. Persistent Gaps and Errors in Reference Databases Impede Ecologically Meaningful Taxonomy Assignments in 18S rRNA Studies: A Case Study of Terrestrial and Marine Nematodes
    Alejandro De Santiago, Tiago José Pereira, Timothy John Ferrero, Natalie Barnes, Delphine Lallias, Simon Creer, and Holly M Bik
    Environmental DNA, 2025

2024

  1. 2024 NMDC Ambassador Training Materials [Slides]
    Julia Mae Kelliher, Francisca Ester Rodriguez, Leah Young Davenport Johnson, Kaelan Jantira Prime, Simon Roux, Emiley A Eloe-Fadrosh, Montana Smith, Alicia Clum, Alejandro De Santiago, Andrian Gajigan, and others
    2024
  2. Soil properties predict below-ground community structure, but not nematode microbiome patterns in semi-arid habitats
    Tiago José Pereira, Alejandro De Santiago, and Holly M Bik
    Molecular Ecology, 2024

2022

  1. Dataset complexity impacts both MOTU delimitation and biodiversity estimates in eukaryotic 18S rRNA metabarcoding studies
    Alejandro De Santiago*, Tiago José Pereira*, Sarah L Mincks, and Holly M Bik
    Environmental DNA, 2022
    * equal contribution

2020

  1. The impact of intragenomic rRNA variation on metabarcoding-derived diversity estimates: A case study from marine nematodes
    Tiago José Pereira, Alejandro De Santiago, Taruna Schuelke, Sarah M Hardy, and Holly M Bik
    Environmental DNA, 2020

2019

  1. Brassicaceae-specific Gretchen Hagen 3 acyl acid amido synthetases conjugate amino acids to chorismate, a precursor of aromatic amino acids and salicylic acid
    Cynthia K Holland, Corey S Westfall, Jason E Schaffer, Alejandro De Santiago, Chloe Zubieta, Sophie Alvarez, and Joseph M Jez
    Journal of Biological Chemistry, 2019
  2. Rhizosphere microbiomes from root knot nematode non-infested plants suppress nematode infection
    Dongmei Zhou, Hui Feng, Taruna Schuelke, Alejandro De Santiago, Qimeng Zhang, Jinfeng Zhang, Chuping Luo, and Lihui Wei
    Microbial Ecology, 2019